DESCRIPTION MW= 33.3 kDa. Full length nsp16 (2′-O-ribose methyltransferase) from SARS-CoV-2, expressed in E. coli. The protein consists in residues 1-298 (NCBI reference sequence YP_009725311.1).
SEQUENCE Numeration of the nsp16 from NCBI YP_009725311.1
10 20 30 40 50 60 SSQAWQPGVA MPNLYKMQRM LLEKCDLQNY GDSATLPKGI MMNVAKYTQL CQYLNTLTLA 70 80 90 100 110 120 VPYNMRVIHF GAGSDKGVAP GTAVLRQWLP TGTLLVDSDL NDFVSDADST LIGDCATVHT 130 140 150 160 170 180 ANKWDLIISD MYDPKTKNVT KENDSKEGFF TYICGFIQQK LALGGSVAIK ITEHSWNADL 190 200 210 220 230 240 YKLMGHFAWW TAFVTNVNAS SSEAFLIGCN YLGKPREQID GYVMHANYIF WRNTNPIQLS 250 260 270 280 290 SYSLFDMSKF PLKLRGTAVM SLKEGQINDM ILSLLSKGRL IIRENNRVVI SSDVLVNN
ISOTOPIC ENRICHMENT none
SUPPLIED AS Protein solution. The concentration is calculated from the absorbance at 280nm (ε280 = 56630 M-1cm-1).
STORAGE -20°C. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
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