DESCRIPTION MW= 25.4 kDa. 206-426 domain of nsp3 (ADP ribose phosphatase, nsp3 macro x domain) from SARS-CoV-2, expressed in E. coli. The protein consists in residues 206-426 (NCBI reference sequence YP_009725299.1) with an additional C-term his-tag (highlighted in italic in the scheme below).
SEQUENCE Numeration of the nsp3 from NCBI YP_009725299.1
210 220 230 240 250 260 M-EVNSF SGYLKLTDNV YIKNADIVEE AKKVKPTVVV NAANVYLKHG GGVAGALNKA 270 280 290 300 310 320 TNNAMQVESD DYIATNGPLK VGGSCVLSGH NLAKHCLHVV GPNVNKGEDI QLLKSAYENF 330 340 350 360 370 380 NQHEVLLAPL LSAGIFGADP IHSLRVCVDT VRTNVYLAVF DKNLYDKLVS SFLEMKSEKQ 390 400 410 420 VEQKIAEIPK EEVKPFITES KPSVEQRKQD DKKIKACVEE VTTTLE LEHHHHHH
ISOTOPIC ENRICHMENT none
SUPPLIED AS Protein solution. The concentration is calculated from the absorbance at 280nm (ε280 = 10680 M-1cm-1).
STORAGE -20°C. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
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